MersV1, Main, Exploration, bibRecord, 003426

Production of shotgun libraries using random amplification.

Identifieur interne : 003426 ( Main/Exploration ); précédent : 003425; suivant : 003427

Production of shotgun libraries using random amplification.

Auteurs : F. Rohwer [États-Unis] ; V. Seguritan ; D H Choi ; A M Segall ; F. Azam

Source :

BioTechniques [ 0736-6205 ] ; 2001.

RBID : pubmed:11464504

Descripteurs français

KwdFr :
- Amorces ADN, Banque de gènes, Chromosomes artificiels de bactérie, Chromosomes artificiels de levure, Coliphages (génétique), Cosmides, Réaction de polymérisation en chaîne ().
MESH :
- génétique : Coliphages.
- Amorces ADN, Banque de gènes, Chromosomes artificiels de bactérie, Chromosomes artificiels de levure, Cosmides, Réaction de polymérisation en chaîne.

English descriptors

KwdEn :
- Chromosomes, Artificial, Bacterial, Chromosomes, Artificial, Yeast, Coliphages (genetics), Cosmids, DNA Primers, Gene Library, Polymerase Chain Reaction (methods).
MESH :
- chemical : DNA Primers.
- genetics : Coliphages.
- methods : Polymerase Chain Reaction.
- Chromosomes, Artificial, Bacterial, Chromosomes, Artificial, Yeast, Cosmids, Gene Library.

Abstract

In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA. RASLs contained inserts from throughout a target genome in an unbiased fashion and did not appear to contain chimeric sequences. This protocol should be useful for shotgun sequencing the genomes of unculturable organisms and rapidly producing shotgun libraries from cosmids, fosmids, yeast artificial chromosomes (YACs), and bacterial artificial chromosomes (BACs).

DOI: 10.2144/01311rr02
PubMed: 11464504

Affiliations:

Le document en format XML

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<author><name sortKey="Choi, D H" sort="Choi, D H" uniqKey="Choi D" first="D H" last="Choi">D H Choi</name>
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<author><name sortKey="Segall, A M" sort="Segall, A M" uniqKey="Segall A" first="A M" last="Segall">A M Segall</name>
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<author><name sortKey="Azam, F" sort="Azam, F" uniqKey="Azam F" first="F" last="Azam">F. Azam</name>
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<series><title level="j">BioTechniques</title>
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<term>Cosmids</term>
<term>DNA Primers</term>
<term>Gene Library</term>
<term>Polymerase Chain Reaction (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN</term>
<term>Banque de gènes</term>
<term>Chromosomes artificiels de bactérie</term>
<term>Chromosomes artificiels de levure</term>
<term>Coliphages (génétique)</term>
<term>Cosmides</term>
<term>Réaction de polymérisation en chaîne ()</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coliphages</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coliphages</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
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<keywords scheme="MESH" xml:lang="en"><term>Chromosomes, Artificial, Bacterial</term>
<term>Chromosomes, Artificial, Yeast</term>
<term>Cosmids</term>
<term>Gene Library</term>
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<term>Banque de gènes</term>
<term>Chromosomes artificiels de bactérie</term>
<term>Chromosomes artificiels de levure</term>
<term>Cosmides</term>
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<front><div type="abstract" xml:lang="en">In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA. RASLs contained inserts from throughout a target genome in an unbiased fashion and did not appear to contain chimeric sequences. This protocol should be useful for shotgun sequencing the genomes of unculturable organisms and rapidly producing shotgun libraries from cosmids, fosmids, yeast artificial chromosomes (YACs), and bacterial artificial chromosomes (BACs).</div>
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<name sortKey="Segall, A M" sort="Segall, A M" uniqKey="Segall A" first="A M" last="Segall">A M Segall</name>
<name sortKey="Seguritan, V" sort="Seguritan, V" uniqKey="Seguritan V" first="V" last="Seguritan">V. Seguritan</name>
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<country name="États-Unis"><region name="Californie"><name sortKey="Rohwer, F" sort="Rohwer, F" uniqKey="Rohwer F" first="F" last="Rohwer">F. Rohwer</name>
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Serveur d'exploration MERS

Production of shotgun libraries using random amplification.

Production of shotgun libraries using random amplification.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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